JASPAR LLM-extracted TF targets

TF → Target relations

Please be aware that these associations were extracted using large language models (LLMs).
Modifiers Transcription factor Target Relation type Text PMID
PGRH19Negative RegulationInterestingly, the same experiments performed in BT-20 cell line provided very similar results as those obtained in MCF-7 cells, with a clear down-regulation mediated by PR-A on the H19 promoter.10442637
SREBF1APOA2BindingOverall, the findings suggest that the wild-type SREBP-1 can bind and transactivate efficiently the apoA-II promoter in cell culture.10364076
SREBF1APOA2Positive RegulationOverall, the findings suggest that the wild-type SREBP-1 can bind and transactivate efficiently the apoA-II promoter in cell culture.10364076
E2F1CDKN2ANeutral RegulationThe E2F1 target gene critical for the senescence response appeared to be the p14(ARF) tumor suppressor.10594030
IncreasedPDX1INSPositive RegulationThese results suggest that high glucose increased DNA-binding activity of PDX-1 by activating atypical PKC including PKC zeta, resulting in transcriptional activation of the human insulin gene promoter.10426567
SP1Serpinh1BindingThese results suggest that two separate elements are necessary for the cell type-specific expression of the hsp47 gene; one is a putative Sp1-binding site at -210 bp necessary for basal expression, and the other is a 500-bp region within the first intron, required for cell type-specific expression.10585450
SP1Serpinh1Positive RegulationThese results suggest that two separate elements are necessary for the cell type-specific expression of the hsp47 gene; one is a putative Sp1-binding site at -210 bp necessary for basal expression, and the other is a 500-bp region within the first intron, required for cell type-specific expression.10585450
FOSL1ElnBindingOverall the results suggest that bFGF represses elastin gene transcription by increasing the amount of the Fra 1 that subsequently binds to the -564- to -558-bp as a heterodimer with c-Jun to form an inhibitory complex.10559225
JUNElnBindingOverall the results suggest that bFGF represses elastin gene transcription by increasing the amount of the Fra 1 that subsequently binds to the -564- to -558-bp as a heterodimer with c-Jun to form an inhibitory complex.10559225
FOSL1ElnNegative RegulationOverall the results suggest that bFGF represses elastin gene transcription by increasing the amount of the Fra 1 that subsequently binds to the -564- to -558-bp as a heterodimer with c-Jun to form an inhibitory complex.10559225
JUNElnNegative RegulationOverall the results suggest that bFGF represses elastin gene transcription by increasing the amount of the Fra 1 that subsequently binds to the -564- to -558-bp as a heterodimer with c-Jun to form an inhibitory complex.10559225
FOXE1TPOPositive RegulationThis was confirmed by transfection experiments and by protein-DNA interaction studies, which demonstrated that CTF/NF1 proteins bind 10 base pairs upstream of the TTF-2-binding site to enhance its action in hormone-induced expression of the TPO gene.10329730
FOXE1TPOBindingThis was confirmed by transfection experiments and by protein-DNA interaction studies, which demonstrated that CTF/NF1 proteins bind 10 base pairs upstream of the TTF-2-binding site to enhance its action in hormone-induced expression of the TPO gene.10329730
NR0B1StarNegative RegulationThese results imply that DAX-1 has an important role in regulating ovarian steroidogenesis by repressing StAR transcription.10403575
TP53BAXPositive RegulationTogether, these data suggest a potential mechanism for the differential regulation of p53-dependent transactivation of the bax and p21 genes.10559267
TP53CDKN1APositive RegulationTogether, these data suggest a potential mechanism for the differential regulation of p53-dependent transactivation of the bax and p21 genes.10559267
MITFTYRNeutral RegulationMITF (microphthalmia-associated transcription factor) is a basic-helix-loop-helix-leucine zipper (bHLHZip) factor which regulates expression of tyrosinase and other melanocytic genes via a CATGTG promoter sequence, and is involved in melanocyte differentiation.10587587
MITFTYRBindingMITF (microphthalmia-associated transcription factor) is a basic-helix-loop-helix-leucine zipper (bHLHZip) factor which regulates expression of tyrosinase and other melanocytic genes via a CATGTG promoter sequence, and is involved in melanocyte differentiation.10587587
IncreasedNR2F2AGTNegative RegulationCo-transfection of Arp-1 reduces estrogen induced promoter activity of reporter constructs containing human angiotensinogen gene promoter.10221773
MutantTP53BAG1Positive RegulationFurthermore, the data suggested that overexpression of BAG-1 in some tumors may be due to upregulation of the human BAG-1 promoter by mutant p53.10467399
PAX6L1CAMPositive RegulationA binding site for homeodomain and Pax proteins is necessary for L1 cell adhesion molecule gene expression by Pax-6 and bone morphogenetic proteins.10051657
ELF5BPIFA2Positive RegulationThus, ESE-2, but not ESE-1, transactivates the parotid gland-specific PSP promoter and the prostate-specific PSA promoter.10506207
ELF5KLK3Positive RegulationThus, ESE-2, but not ESE-1, transactivates the parotid gland-specific PSP promoter and the prostate-specific PSA promoter.10506207
ZNF148COL1A2BindingHere we show that another zinc-finger transcription factor, BFCOL1, which binds to the proximal proalpha2(I) collagen promoter, could also bind to this GC-box of the p21 promoter.10066455
ZNF148CDKN1ABindingHere we show that another zinc-finger transcription factor, BFCOL1, which binds to the proximal proalpha2(I) collagen promoter, could also bind to this GC-box of the p21 promoter.10066455
IncreasedJUNTNFPositive RegulationForced expression of c-Jun in ATF2-expressing melanoma cells restored TNFalpha expression, suggesting that both forms of ATF2 sequestered transcription factors that positively regulate TNFalpha expression in response to UV irradiation.10318823
ATF2TNFNegative RegulationForced expression of c-Jun in ATF2-expressing melanoma cells restored TNFalpha expression, suggesting that both forms of ATF2 sequestered transcription factors that positively regulate TNFalpha expression in response to UV irradiation.10318823
RELAABCB1Negative RegulationNegative regulation of MDR1 promoter activity in MCF-7, but not in multidrug resistant MCF-7/Adr, cells by cross-coupled NF-kappa B/p65 and c-Fos transcription factors and their interaction with the CAAT region.10026303
FOSABCB1Negative RegulationNegative regulation of MDR1 promoter activity in MCF-7, but not in multidrug resistant MCF-7/Adr, cells by cross-coupled NF-kappa B/p65 and c-Fos transcription factors and their interaction with the CAAT region.10026303
RELAABCB1BindingNegative regulation of MDR1 promoter activity in MCF-7, but not in multidrug resistant MCF-7/Adr, cells by cross-coupled NF-kappa B/p65 and c-Fos transcription factors and their interaction with the CAAT region.10026303
FOSABCB1BindingNegative regulation of MDR1 promoter activity in MCF-7, but not in multidrug resistant MCF-7/Adr, cells by cross-coupled NF-kappa B/p65 and c-Fos transcription factors and their interaction with the CAAT region.10026303
Tp53PcnaNeutral RegulationWe found that exogenous p53 was unable to mimic the UV irradiation to induce rat PCNA promoter and that the UV induction of the rat PCNA promoter was seen in p53 deficient cells.10321841
ETS2BCL2L1Positive RegulationThe bcl-x promoter contains potential Ets binding sites, and we show that the transcription factor, Ets2, first identified by its sequence identity to v-ets of the E26 retrovirus, can transactivate the bcl-x promoter.10082528
NR5A1SCARB1Positive RegulationThe results confirm that indeed both SF-1 and SREBP-1a synergize to induce HDL receptor SR-BI gene expression.10579331
SREBF1SCARB1Positive RegulationThe results confirm that indeed both SF-1 and SREBP-1a synergize to induce HDL receptor SR-BI gene expression.10579331
FOXO3FASLGPositive RegulationWithin the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene.10102273
CEBPAPRLPositive RegulationExpression of C/EBP alpha increased basal PRL gene expression almost 6-fold, whereas expression of Chop10 that can act as an inhibitor of C/EBP alpha reduced the basal activity of the PRL promoter 60-75%.10499509
DDIT3PRLNegative RegulationExpression of C/EBP alpha increased basal PRL gene expression almost 6-fold, whereas expression of Chop10 that can act as an inhibitor of C/EBP alpha reduced the basal activity of the PRL promoter 60-75%.10499509
STAT3CDKN1ABindingIL-6 dependent upregulation of p21WAF1/CIP1 was associated with binding of both STAT3 and STAT1 to the p21WAF1/CIP1 promoter.10023678
STAT1CDKN1ABindingIL-6 dependent upregulation of p21WAF1/CIP1 was associated with binding of both STAT3 and STAT1 to the p21WAF1/CIP1 promoter.10023678
SPI1NCF2Positive RegulationIn these studies, we identify an NCF2 cis element, necessary for IFN-gamma-induced p67phox expression, and determine that this element is activated by cooperation between the transcription factors PU.1, IFN regulatory factor 1 (IRF1), and the IFN consensus-binding protein (ICSBP).10570299
IRF1NCF2Positive RegulationIn these studies, we identify an NCF2 cis element, necessary for IFN-gamma-induced p67phox expression, and determine that this element is activated by cooperation between the transcription factors PU.1, IFN regulatory factor 1 (IRF1), and the IFN consensus-binding protein (ICSBP).10570299
IRF8NCF2Positive RegulationIn these studies, we identify an NCF2 cis element, necessary for IFN-gamma-induced p67phox expression, and determine that this element is activated by cooperation between the transcription factors PU.1, IFN regulatory factor 1 (IRF1), and the IFN consensus-binding protein (ICSBP).10570299
YY1IL3Neutral RegulationRegulation of a cell type-specific silencer in the human interleukin-3 gene promoter by the transcription factor YY1 and an AP2 sequence-recognizing factor.10480868
IncreasedEGR1Me1Negative RegulationThese results suggest that insulin induces a quick, transient, and Ras/MAP kinase-dependent activation of Egr-1 which leads to a transient repression of ME gene transcription.10364249
IncreasedMYCCDKN1ANegative RegulationWe show here that exogenous c-Myc inhibits p21 expression in SkBr3 and LNCaP cells induced to enter into S-phase.10319992
IncreasedELK4EGR1Positive RegulationCoexpression of Sap-1a and Elk-1 resulted in a marked increase in GH induction of egr-1 promoter activity, although transfection with expression vectors for either Ets factor alone did not significantly enhance the GH response.10194767
IncreasedELK1EGR1Positive RegulationCoexpression of Sap-1a and Elk-1 resulted in a marked increase in GH induction of egr-1 promoter activity, although transfection with expression vectors for either Ets factor alone did not significantly enhance the GH response.10194767
IncreasedELK4EGR1Neutral RegulationCoexpression of Sap-1a and Elk-1 resulted in a marked increase in GH induction of egr-1 promoter activity, although transfection with expression vectors for either Ets factor alone did not significantly enhance the GH response.10194767
IncreasedELK1EGR1Neutral RegulationCoexpression of Sap-1a and Elk-1 resulted in a marked increase in GH induction of egr-1 promoter activity, although transfection with expression vectors for either Ets factor alone did not significantly enhance the GH response.10194767
CEBPAPRLNeutral RegulationThese data suggest that C/EBP alpha is an important transcription factor that regulates PRL gene expression.10499509
EGR3FASLGPositive RegulationThe transcription factors NF-AT and Egr-3 (a member of the immediate-early transcription factors involved in cellular growth and differentiation) have been implicated in activation of the CD95L promoter upon T cell activation.10508276
IRF1IRF2Positive RegulationIRF-1 also induced expression of its homologous repressor IRF-2 as indicated by EMSA analysis using an IRF-E probe from the IRF-2 promoter; and by cotransfection of IRF-1 together with an IRF-2 promoter CAT construct.10321737
IRF1IRF2BindingIRF-1 also induced expression of its homologous repressor IRF-2 as indicated by EMSA analysis using an IRF-E probe from the IRF-2 promoter; and by cotransfection of IRF-1 together with an IRF-2 promoter CAT construct.10321737
RbpjMYOD1Negative RegulationDelta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis.10066785
EGR1F3BindingAs shown by immunofluorescence and electrophoretic mobility shift assays, upon VEGF treatment EGR-1 rapidly accumulates in the nucleus and binds to its respective recognition site in the tissue factor promoter.10339488
RESTGRIA2Negative RegulationWe provide multiple lines of evidence that the N-terminal domain of REST represses transcription of the GluR2 and type II sodium-channel genes by recruiting the corepressor Sin3A and histone deacetylase (HDAC) to the promoter region in nonneuronal cells.10491605
RESTSCN2ANegative RegulationWe provide multiple lines of evidence that the N-terminal domain of REST represses transcription of the GluR2 and type II sodium-channel genes by recruiting the corepressor Sin3A and histone deacetylase (HDAC) to the promoter region in nonneuronal cells.10491605
ZEB1CD4BindingWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
ZEB1CD4Negative RegulationWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
TCF3CD4BindingWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
TCF3CD4Positive RegulationWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
Tcf12CD4BindingWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
Tcf12CD4Positive RegulationWe show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells.10508188
WT1ODC1Negative RegulationSimultaneous mutation of the WT1-binding motif and the two E-boxes completely abolished inhibition by WT1 of ODC promoter activity.10047468
WT1ODC1BindingSimultaneous mutation of the WT1-binding motif and the two E-boxes completely abolished inhibition by WT1 of ODC promoter activity.10047468
IncreasedARKLK3BindingIn addition, when nuclear AR from forskolin-treated LNCaP cells was incubated with oligonucleotides encoding an androgen response element of the PSA promoter and examined by electromobility shift assay, an increase in AR-androgen response element complex formation was observed.10075669
IncreasedSTAT6IRF1Negative RegulationIn a cell line with low endogenous Stat6, increasing levels of activated Stat6 at constant doses of IFN-gamma and IL-4 leads to inhibition of the IRF-1 promoter.10528163
TP53ALBNegative RegulationWe show that p53 wild type and tumor-derived p53 mutations repress C/EBP-mediated transactivation of the albumin promoter.10542249
MutantTP53ALBNegative RegulationWe show that p53 wild type and tumor-derived p53 mutations repress C/EBP-mediated transactivation of the albumin promoter.10542249
Irf1CiitaPositive RegulationDifferential selectivity of CIITA promoter activation by IFN-gamma and IRF-1 in astrocytes and macrophages: CIITA promoter activation is not affected by TNF-alpha.10505975
Irf1CiitaPositive RegulationDifferential selectivity of CIITA promoter activation by IFN-gamma and IRF-1 in astrocytes and macrophages: CIITA promoter activation is not affected by TNF-alpha.10505975
Irf1CiitaPositive RegulationDifferential selectivity of CIITA promoter activation by IFN-gamma and IRF-1 in astrocytes and macrophages: CIITA promoter activation is not affected by TNF-alpha.10505975
Irf1CiitaPositive RegulationDifferential selectivity of CIITA promoter activation by IFN-gamma and IRF-1 in astrocytes and macrophages: CIITA promoter activation is not affected by TNF-alpha.10505975
IncreasedMYCCCND2Positive RegulationAddition of trichostatin A to quiescent cells mimics activation of Myc and induces cyclin D2 expression, suggesting that cyclin D2 is repressed in a histone deacetylase-dependent manner in quiescent cells.10508165
Stat6SELPPositive RegulationThe sustained activation of Stat6 induced by IL-4 is likely to be a key factor leading to the prolonged activation of the P-selectin promoter, thereby resulting in prolonged P-selectin upregulation.10364072
TCF7L2MMP7BindingThe matrilysin promoter is upregulated as much as 12-fold by beta-catenin in colon tumor cell lines in a manner inversely proportional to the endogenous levels of beta-catenin/Tcf complex and is dependent upon a single optimal Tcf-4 recognition site.10362259
TCF7L2MMP7Positive RegulationThe matrilysin promoter is upregulated as much as 12-fold by beta-catenin in colon tumor cell lines in a manner inversely proportional to the endogenous levels of beta-catenin/Tcf complex and is dependent upon a single optimal Tcf-4 recognition site.10362259
GATA1SPTBBindingUsing transfection of promoter/reporter plasmids in human tissue culture cell lines, in vitro DNase I footprinting analyses, and gel mobility shift assays, a beta-spectrin gene erythroid promoter with two binding sites for GATA-1 and one site for CACCC-related proteins was identified.10037687
MutantJUNCCL5Negative RegulationA c-Jun mutant which lacks a transcriptional activation domain interfered in the activation of the RANTES promoter.10075858
CEBPBCEBPABindingThis decrease in Sp1 level early in the differentiation program appears to facilitate access of C/EBPbeta and/or C/EBPdelta to the C/EBP regulatory element and, thereby, derepression of the C/EBPalpha gene.10373535
CEBPDCEBPABindingThis decrease in Sp1 level early in the differentiation program appears to facilitate access of C/EBPbeta and/or C/EBPdelta to the C/EBP regulatory element and, thereby, derepression of the C/EBPalpha gene.10373535
CEBPBCEBPAPositive RegulationThis decrease in Sp1 level early in the differentiation program appears to facilitate access of C/EBPbeta and/or C/EBPdelta to the C/EBP regulatory element and, thereby, derepression of the C/EBPalpha gene.10373535
CEBPDCEBPAPositive RegulationThis decrease in Sp1 level early in the differentiation program appears to facilitate access of C/EBPbeta and/or C/EBPdelta to the C/EBP regulatory element and, thereby, derepression of the C/EBPalpha gene.10373535
ReducedSP1CEBPANegative RegulationThis decrease in Sp1 level early in the differentiation program appears to facilitate access of C/EBPbeta and/or C/EBPdelta to the C/EBP regulatory element and, thereby, derepression of the C/EBPalpha gene.10373535
MYCARBindingMultiple androgen response elements and a Myc consensus site in the androgen receptor (AR) coding region are involved in androgen-mediated up-regulation of AR messenger RNA.10551783
ETS1FLT1Positive RegulationIn vitro data also point to a role of Ets-1 as a transcriptional activator of Flt-1.10554042
IncreasedEsr2PrlPositive RegulationWe conclude that 1) both ER beta and TERP messenger RNAs in GH3 cells are increased by estradiol in a dose- and time-dependent manner, whereas ER alpha is not altered; 2) a 58-kDa ER beta protein is expressed in both the pituitary and GH3 cells; and 3) overexpression of ER beta increases estrogen-induced PRL gene expression.10342855
PrebPrlPositive RegulationTransient transfection employing PREB-negative C6 rat glial cells showed that PREB is as active as, and additive with, Pit-1 in transactivation of a PRL promoter construct, and that PREB, but not Pit-1, can mediate transcriptional activation by protein kinase A (PKA).10194769
Pou1f1PrlPositive RegulationTransient transfection employing PREB-negative C6 rat glial cells showed that PREB is as active as, and additive with, Pit-1 in transactivation of a PRL promoter construct, and that PREB, but not Pit-1, can mediate transcriptional activation by protein kinase A (PKA).10194769
STAT1Socs3BindingA STAT-1/STAT-3 binding element, located at nucleotides -72 to -64, was essential for LIF stimulation of SOCS-3 promoter activity.10359822
STAT3Socs3BindingA STAT-1/STAT-3 binding element, located at nucleotides -72 to -64, was essential for LIF stimulation of SOCS-3 promoter activity.10359822
Pou2f1Ly6eBindingThe complexity of IFN-mediated regulation of the murine Ly-6E gene in T cell lines is highlighted by the following observations: 1) multiple regulatory regions are present within different parts of the Ly-6E promoter and are necessary for IFN inducibility of the Ly-6E gene, 2) multiple transcription factors including Oct-1 and Oct-2 and the high mobility group (HMG) protein HMGI(Y) bind to regulatory elements present within the G region required for both IFN-alphabeta and IFN-gamma responses, 3) mutational analysis of the G region reveals that a complex interaction exists between the factors binding to this region as shown by their mutual interdependence for detection in DMSA, and 4) inhibition of expression of HMG proteins by antisense HMGI-C RNA in EL4 cells causes the loss of IFN-alphabeta and IFN-gamma inducibility of the endogenous Ly-6 gene.10395674
Pou2f2Ly6eBindingThe complexity of IFN-mediated regulation of the murine Ly-6E gene in T cell lines is highlighted by the following observations: 1) multiple regulatory regions are present within different parts of the Ly-6E promoter and are necessary for IFN inducibility of the Ly-6E gene, 2) multiple transcription factors including Oct-1 and Oct-2 and the high mobility group (HMG) protein HMGI(Y) bind to regulatory elements present within the G region required for both IFN-alphabeta and IFN-gamma responses, 3) mutational analysis of the G region reveals that a complex interaction exists between the factors binding to this region as shown by their mutual interdependence for detection in DMSA, and 4) inhibition of expression of HMG proteins by antisense HMGI-C RNA in EL4 cells causes the loss of IFN-alphabeta and IFN-gamma inducibility of the endogenous Ly-6 gene.10395674
Hmga1Ly6eBindingThe complexity of IFN-mediated regulation of the murine Ly-6E gene in T cell lines is highlighted by the following observations: 1) multiple regulatory regions are present within different parts of the Ly-6E promoter and are necessary for IFN inducibility of the Ly-6E gene, 2) multiple transcription factors including Oct-1 and Oct-2 and the high mobility group (HMG) protein HMGI(Y) bind to regulatory elements present within the G region required for both IFN-alphabeta and IFN-gamma responses, 3) mutational analysis of the G region reveals that a complex interaction exists between the factors binding to this region as shown by their mutual interdependence for detection in DMSA, and 4) inhibition of expression of HMG proteins by antisense HMGI-C RNA in EL4 cells causes the loss of IFN-alphabeta and IFN-gamma inducibility of the endogenous Ly-6 gene.10395674
SP3CDKN1APositive RegulationTaken together, these results suggest that histone deacetylase inhibitor up-regulates p21/WAF1/Cip1 transcription by Sp3 but not by Sp1.10485470
IncreasedOtx2NCAM1Positive RegulationIn addition, transient expression of Otx2 in COS7 cells is able to dramatically enhance the transcriptional activity of the NCAM promoter.10216227
CTCFAPPPositive RegulationThese results suggest that CTCF is pivotal for the up-regulation of APP expression during synaptogenesis in primary neurons.10582586
JUNCCND1Bindingp300 activated the cyclin D1 promoter through an activator protein-1 (AP-1) site at -954 and was identified within a DNA-bound complex with c-Jun at the AP-1 site.10567390
PGRIRS2Positive RegulationOur analysis of the regulation of IRS-2 in HeLa cell models shows that synergistic induction of IRS-2 by cAMP and RU486 can be mediated by progesterone receptors (PR) and glucocorticoid receptors (GR) and occurs through a relative slow mechanism that requires ongoing protein synthesis.10077005
NR3C1IRS2Positive RegulationOur analysis of the regulation of IRS-2 in HeLa cell models shows that synergistic induction of IRS-2 by cAMP and RU486 can be mediated by progesterone receptors (PR) and glucocorticoid receptors (GR) and occurs through a relative slow mechanism that requires ongoing protein synthesis.10077005
Sp1FASLGBindingNuclear extracts of Sertoli cells contain Sp1 and Sp3 that specifically binds to the Sp1 motif present in the FasL gene, and overexpression of Sp1 but not Sp3 leads to a further increase of transcription from the FasL promoter-enhancer region.10075666
SP3FASLGBindingNuclear extracts of Sertoli cells contain Sp1 and Sp3 that specifically binds to the Sp1 motif present in the FasL gene, and overexpression of Sp1 but not Sp3 leads to a further increase of transcription from the FasL promoter-enhancer region.10075666
IncreasedSp1FASLGPositive RegulationNuclear extracts of Sertoli cells contain Sp1 and Sp3 that specifically binds to the Sp1 motif present in the FasL gene, and overexpression of Sp1 but not Sp3 leads to a further increase of transcription from the FasL promoter-enhancer region.10075666
ATF2TNFNegative RegulationTaken together, our findings suggest that ATF2 contributes to UVC-induced apoptosis through transcriptional silencing of TNFalpha, which balances Fas-mediated cell death in melanoma.10318823
PPARANR1D1Positive RegulationTransfection studies with reporter constructs driven by the human Rev-erbalpha promoter revealed that fibrates induce Rev-erbalpha expression at the transcriptional level via PPARalpha.10076997
SP1MYBL2BindingWhen the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results.10022815
MYBL2MYBL2Positive RegulationWhen the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results.10022815
SP1CDKN1APositive RegulationTaken together, these results suggest that Sp1 or Sp1-related proteins mediate FB1-induced activation of the p21 promoter.10212208
NR2F1EGR1Positive RegulationIn fact, the coactivators p300 and steroid receptor activator 1 can enhance the transactivation of the NGFI-A promoter induced by COUP-TFI.10082539
NRF1CXCR4Positive RegulationHTLV type I Tax activation of the CXCR4 promoter by association with nuclear respiratory factor 1.10381170
MAXCCND2Negative RegulationThe cyclin D2 promoter is repressed by Mad-Max complexes and de-repressed by Myc via a single highly conserved E-box element.10508165
MYCCCND2Positive RegulationThe cyclin D2 promoter is repressed by Mad-Max complexes and de-repressed by Myc via a single highly conserved E-box element.10508165
ReducedTfcp2Hba-a2Positive RegulationDuring HMBA-induced terminal differentiation of antisense CP2 expressing MEL cells, the transcription of endogenous alpha-globin gene was suppressed as expected.10491335
ReducedTfcp2Hba-a1Positive RegulationDuring HMBA-induced terminal differentiation of antisense CP2 expressing MEL cells, the transcription of endogenous alpha-globin gene was suppressed as expected.10491335
CDX2GCGNeutral RegulationFurther, we found that the presence of cdx-2 enhanced the interaction of pax-6 with p300, thus establishing a molecular complex of transcription factors implicated in tissue-specific glucagon gene expression with the basal transcriptional machinery.10506141
PAX6GCGNeutral RegulationFurther, we found that the presence of cdx-2 enhanced the interaction of pax-6 with p300, thus establishing a molecular complex of transcription factors implicated in tissue-specific glucagon gene expression with the basal transcriptional machinery.10506141
IncreasedCREB1IL10Neutral RegulationOur data suggest that intracellular cAMP may directly affect expression of the immunoregulatory cytokine IL-10 in monocytic cells via activation of the eukaryotic transcription factors CREB-1 and ATF-1 and their binding to CRE1 and CRE4 in the upstream enhancer of the IL-10 promoter.10540320
IncreasedATF1IL10Neutral RegulationOur data suggest that intracellular cAMP may directly affect expression of the immunoregulatory cytokine IL-10 in monocytic cells via activation of the eukaryotic transcription factors CREB-1 and ATF-1 and their binding to CRE1 and CRE4 in the upstream enhancer of the IL-10 promoter.10540320
IncreasedCREB1IL10BindingOur data suggest that intracellular cAMP may directly affect expression of the immunoregulatory cytokine IL-10 in monocytic cells via activation of the eukaryotic transcription factors CREB-1 and ATF-1 and their binding to CRE1 and CRE4 in the upstream enhancer of the IL-10 promoter.10540320
IncreasedATF1IL10BindingOur data suggest that intracellular cAMP may directly affect expression of the immunoregulatory cytokine IL-10 in monocytic cells via activation of the eukaryotic transcription factors CREB-1 and ATF-1 and their binding to CRE1 and CRE4 in the upstream enhancer of the IL-10 promoter.10540320
MYCTERTPositive RegulationSince telomerase activation frequently correlates with immortalization and telomerase functions to stabilize telomers in cycling cells, we tested whether Myc-induced activation of TERT gene expression represents an important mechanism through which c-Myc acts to immortalize cells.10022128
MycTertPositive RegulationEmploying the rat embryo fibroblast cooperation assay, we show that TERT is unable to substitute for c-Myc in the transformation of primary rodent fibroblasts, suggesting that the transforming activities of Myc extend beyond its ability to activate TERT gene expression and hence telomerase activity.10022128
ESR1H19Positive RegulationWe demonstrated that ERalpha up-regulated the H19 promoter in MCF-7 and in HBL-100, whereas PR-A did not have any effect per se.10442637
CebpbTFAP2ABindingThis novel regulatory scheme involves: (1) unique gradients of expression for each transcription factor, i.e., C/EBPbeta and AP-2 most abundant in the lower epidermis, C/EBPalpha in the upper; (2) C/EBP-binding sites in the ap-2alpha gene promoter, through which C/EBPbeta stimulates ap-2alpha; and (3) AP-2 binding sites in the c/ebpalpha promoter, through which AP-2 represses c/ebpalpha.10588870
CebpbTFAP2APositive RegulationThis novel regulatory scheme involves: (1) unique gradients of expression for each transcription factor, i.e., C/EBPbeta and AP-2 most abundant in the lower epidermis, C/EBPalpha in the upper; (2) C/EBP-binding sites in the ap-2alpha gene promoter, through which C/EBPbeta stimulates ap-2alpha; and (3) AP-2 binding sites in the c/ebpalpha promoter, through which AP-2 represses c/ebpalpha.10588870
SRFFOSPositive RegulationThe SRF and bHLH proteins appear to bind to the SRE and activate the c-fos promoter in Sertoli cells.10319327
MutantSP1CGB3Positive RegulationHowever, cAMP stimulation of the CGbeta promoter is reduced by AP-2 mutations, whereas Sp1 mutations enhance cAMP activation.10551788
NFKB1NFKBIABindingProlonged NF-kappaB binding and a temporal switch in the composition of NF-kappaB complexes bound to the -63 to -53 kappaB1 site of the IkappaBalpha promoter were also observed; with time after induction, decreased levels of transcriptionally active p50-p65 and increased p50-c-Rel heterodimers were detected at the kappaB1 site.10454561
ReducedRELANFKBIABindingProlonged NF-kappaB binding and a temporal switch in the composition of NF-kappaB complexes bound to the -63 to -53 kappaB1 site of the IkappaBalpha promoter were also observed; with time after induction, decreased levels of transcriptionally active p50-p65 and increased p50-c-Rel heterodimers were detected at the kappaB1 site.10454561
IncreasedRELNFKBIABindingProlonged NF-kappaB binding and a temporal switch in the composition of NF-kappaB complexes bound to the -63 to -53 kappaB1 site of the IkappaBalpha promoter were also observed; with time after induction, decreased levels of transcriptionally active p50-p65 and increased p50-c-Rel heterodimers were detected at the kappaB1 site.10454561
MycGCFC2Positive RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
JUNGCFC2Positive RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
FOSGCFC2Positive RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
MycFN1Negative RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
JUNFN1Negative RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
FOSFN1Negative RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
GCFC2FN1Negative RegulationThese results indicate that the G10BP-1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP-1 during G1-to-S phase progression.10421838
CEBPEELANENeutral RegulationThese results demonstrate a role for C/EBPepsilon in regulating myeloid promoters, such as neutrophil elastase, probably through a direct interaction with c-myb.10233885
FLI1GP9Positive RegulationIn this study, transient cotransfection of several GPIX promoter/reporter constructs into 293T kidney fibroblasts with a Fli-1 expression vector shows that the oncogenic protein Fli-1 can transactivate the GPIX promoter when an intact GPIX Ets site is present.10194443
IncreasedPpargUcp1Positive RegulationRodent brown adipocytes express peroxisome proliferator activated receptor-alpha (PPARalpha) and PPARgamma and while the rodent uncoupling protein-1 (UCP-1) gene contains a putative peroxisome proliferator response element (PPRE), only PPARgamma activation by thiazolidinediones increase UCP-1 mRNA levels.10529360
IncreasedPpargUcp1Positive RegulationRodent brown adipocytes express peroxisome proliferator activated receptor-alpha (PPARalpha) and PPARgamma and while the rodent uncoupling protein-1 (UCP-1) gene contains a putative peroxisome proliferator response element (PPRE), only PPARgamma activation by thiazolidinediones increase UCP-1 mRNA levels.10529360
DbpCyp2a4Neutral RegulationBiochemical evidence, including in vitro DNase I footprinting on the Cyp2a4 and Cyp2a5 promoters and cotransfection experiments with the human hepatoma cell line HepG2, suggests that the Cyp2a4 and Cyp2a5 genes are indeed regulated by DBP.10490589
DbpCyp2a5Neutral RegulationBiochemical evidence, including in vitro DNase I footprinting on the Cyp2a4 and Cyp2a5 promoters and cotransfection experiments with the human hepatoma cell line HepG2, suggests that the Cyp2a4 and Cyp2a5 genes are indeed regulated by DBP.10490589
Neurod2Itpr1Neutral RegulationFrom these results, we suggest that NDRF is involved in the tissue-specific regulation of IP3R1 gene expression in the CNS.10098882
STAT4IRF1Positive RegulationStrong IL-12-dependent activation of a reporter gene construct containing the IRF-1 IFN-gamma-activated sequence element provides further evidence for the key role of signal transducer and activator of transcription-4 in the IL-12-induced up-regulation of IRF-1 transcripts in T cells.10037767
ReducedPdx1INSBindingImportantly, the lower Pdx-1 protein levels led to a greater than 50% reduction in Pdx-1 binding activity to the A3 element on the insulin gene promoter, and a significant reduction in insulin mRNA levels.10393088
CUX1TGFBR2BindingHuman cut-like repressor protein binds TGFbeta type II receptor gene promoter.10545217
MYBL2COL5A2Negative RegulationA model of indirect repression of the Col5A2 gene by B-Myb, via interaction with a positively-acting matrix regulatory factor, termed MRF-V, is discussed.10429946
SP1CYP11A1Positive RegulationActivator protein-2 mediates transcriptional activation of the CYP11A1 gene by interaction with Sp1 rather than binding to DNA.10446912
NR5A1STARPositive RegulationThus, transcriptional activation of the StAR gene by a steroidogenic factor-1-dependent mechanism may represent a common pathway for ACTH (protein kinase A) and AII action in stimulating steroid production in the adrenal fasciculata and glomerulosa.10499490
PPARGLPLPositive RegulationCotransfection of COUP-TF expression constructs in the renal epithelial 293T cell line significantly increased transcription from the LPL promoter in synergy with PPARgamma2/RXRalpha heterodimers.10098492
RXRALPLPositive RegulationCotransfection of COUP-TF expression constructs in the renal epithelial 293T cell line significantly increased transcription from the LPL promoter in synergy with PPARgamma2/RXRalpha heterodimers.10098492
STAT3HSP90AB1Positive RegulationRecent studies have shown that the cytokine IL-6 activates hsp 90 gene expression via specific transcription factors that include STAT-3 (signal transducer and activator of transcription 3).10447736
SP1LDLRPositive RegulationCo-stimulation of promoter for low density lipoprotein receptor gene by sterol regulatory element-binding protein and Sp1 is specifically disrupted by the yin yang 1 protein.10224053
YY1LDLRNegative RegulationCo-stimulation of promoter for low density lipoprotein receptor gene by sterol regulatory element-binding protein and Sp1 is specifically disrupted by the yin yang 1 protein.10224053
CREB1THNeutral RegulationThese results support the hypothesis that CREB (not other CRE-binding proteins) is the key transcription factor that is required for regulating TH gene expression in response to cAMP.10407170
Stat5aPRLRPositive RegulationOur results suggest that GH and PRL increase the levels of exon 1A- and 1C-containing PRLR mRNA species and furthermore that the transcriptional activity of the 1A promoter is increased via activation of STAT5a and STAT5b.10373481
STAT5BPRLRPositive RegulationOur results suggest that GH and PRL increase the levels of exon 1A- and 1C-containing PRLR mRNA species and furthermore that the transcriptional activity of the 1A promoter is increased via activation of STAT5a and STAT5b.10373481
TP53BAXNeutral RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP53CDKN1ANeutral RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP53CCNG1Neutral RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP53MDM2Neutral RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP73BAXPositive RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP73CDKN1APositive RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP73CCNG1Positive RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
TP73MDM2Positive RegulationOur results show that the large E1B proteins very efficiently inhibited the activity of p53 on the Bax, p21(Waf1), cyclin G, and MDM2 reporter constructs but had no effect on the activation of the same reporter constructs by p73, with the exception of some inhibition of the Bax promoter by Ad12 E1B.10207112
VDRRARBNegative RegulationVitamin D represses retinoic acid-dependent transactivation of the retinoic acid receptor-beta2 promoter: the AF-2 domain of the vitamin D receptor is required for transrepression.10342883
RELABCL2L1Positive RegulationCo-transfection experiment using a bcl-x promoter reporter construct and an expression vector for NF-kappaB p50 or p65 indicates that p65, but not p50, up-regulates the promoter activity of the bcl-x gene.10585435
SPI1NCF1BindingWe conclude that the immediate flanking nucleotides of the PU.1 consensus motif have significant effects on PU.1 binding avidity and activity and that this region is the dominant cis element regulating p47phox expression.10542290
SPI1NCF1Neutral RegulationWe conclude that the immediate flanking nucleotides of the PU.1 consensus motif have significant effects on PU.1 binding avidity and activity and that this region is the dominant cis element regulating p47phox expression.10542290
Runx2Runx2Positive RegulationWe also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized.10215629
Runx2Runx2BindingWe also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized.10215629
CEBPBCEBPBPositive RegulationInterestingly, the C/EBPbeta promoter is not activated by C/EBPalpha, another C/EBP family member highly expressed in myelomonocytic cells, indicating that the autoregulation is specific for C/EBPbeta.10542314
E2f1E2f1Positive RegulationThese results suggest that PARP plays a role in the induction of E2F-1 promoter activity, which then positively regulates both E2F-1 and pol alpha expression, when quiescent cells reenter the cell cycle upon recovery from aphidicolin exposure or removal of serum.10490838
E2f1POLA1Positive RegulationThese results suggest that PARP plays a role in the induction of E2F-1 promoter activity, which then positively regulates both E2F-1 and pol alpha expression, when quiescent cells reenter the cell cycle upon recovery from aphidicolin exposure or removal of serum.10490838
Sp1Cdkn1aPositive RegulationFurthermore, p300 was able to act as a co-activator for Sp1-mediated transcriptional activation in PC12 cells, suggesting that p300 and Sp1 may cooperate in activating p21 transcription during the withdrawal of neuronal precursors from the cell cycle.10362258
IRF1CIITABindingHere we report that the human CIITA Type IV promoter IRF-E binds IRF-1 and can be activated by exogenous expression of IRF-1.10557076
IncreasedIRF1CIITAPositive RegulationHere we report that the human CIITA Type IV promoter IRF-E binds IRF-1 and can be activated by exogenous expression of IRF-1.10557076
IncreasedSrebf1Scarb1Neutral RegulationThe cysteine protease inhibitor N-acetyl-leucyl-leucyl-norleucinal, which inhibits SREBP degradation, enhanced the effect of SREBP-1a on the regulation of the rat HDL receptor SR-BI gene.10579331
SP1PSEN1BindingThus, transfection and footprinting assays correlate to suggest that Sp1 transcription factor(s) bind at several sites upstream and downstream from the initiation site and activate the transcription of the PS1 promoter.10446206
SP1PSEN1Positive RegulationThus, transfection and footprinting assays correlate to suggest that Sp1 transcription factor(s) bind at several sites upstream and downstream from the initiation site and activate the transcription of the PS1 promoter.10446206
NEUROD1AK1Positive RegulationThe CAT reporter assay of the 5'-flanking region of the AK1 gene suggests that NeuroD activates the AK1 expression through E-boxes in the promoter region.10101232
Altered_FunctionRUNX1BCL2Positive RegulationHowever, AML1/ETO can also activate the BCL-2 promoter and cause enhanced hematopoietic progenitor self-renewal in vitro, suggesting gain-of-functions unique to the fusion protein.10208431
ESR1HRASBindingOur data demonstrate a direct (i) interaction of c-Ha-ras sequence with estrogen receptor and (ii) stimulatory effect of estrogen on c-Ha-ras gene transcription and suggest that alteration in transcriptional regulation of c-Ha-ras gene by estrogen may play an important role in progression of breast cancer.10521493
ATF2CCND1BindingWe show that ATF-2 homodimers and ATF-2/c-Jun heterodimers, but not c-Jun homodimers, were able to bind the CRE of the cyclin D1 promoter.10500157
JUNCCND1BindingWe show that ATF-2 homodimers and ATF-2/c-Jun heterodimers, but not c-Jun homodimers, were able to bind the CRE of the cyclin D1 promoter.10500157
MYCTERTPositive RegulationHere, we identify the c-Myc transcription factor as a direct mediator of telomerase activation in primary human fibroblasts through its ability to specifically induce TERT gene expression.10022128
ReducedESRRACYP19A1Positive RegulationBecause toxaphene is not an inhibitor of aromatase, it is thought that the decrease of the aromatase activity in ERR alpha-1 transfected HepG2 cells following toxaphene treatment resulted from a suppression of the aromatase expression by toxaphene acting as the antagonist of ERR alpha-1.10493499
WT1MYCNNegative RegulationHere, we show that the proto-oncogene N-myc promoter was down-regulated by WT1 in transient transfection assays.10470095
CEBPAALBBindingThe binding of C/EBPalpha or -beta to its cognate sequence in the albumin promoter is not inhibited by p53 expression.10542249
CEBPBALBBindingThe binding of C/EBPalpha or -beta to its cognate sequence in the albumin promoter is not inhibited by p53 expression.10542249
RBPJIL6BindingInteraction of the nuclear protein CBF1 with the kappaB site of the IL-6 gene promoter.10373597
NR5A1CYP19A1BindingIn endometriosis, however, an aberrantly expressed factor, SF-1, displaces COUP-TF to bind to this same promoter and activates aromatase expression and thus local estrogen biosynthesis.10593363
NR5A1CYP19A1Positive RegulationIn endometriosis, however, an aberrantly expressed factor, SF-1, displaces COUP-TF to bind to this same promoter and activates aromatase expression and thus local estrogen biosynthesis.10593363
NR2F2CYP19A1BindingIn endometriosis, however, an aberrantly expressed factor, SF-1, displaces COUP-TF to bind to this same promoter and activates aromatase expression and thus local estrogen biosynthesis.10593363
SP1PKMNeutral RegulationFunctional studies by site-directed mutagenesis on the role of Sp1 in the expression of the pyruvate kinase M and aldolase A genes.10023068
SP1ALDOANeutral RegulationFunctional studies by site-directed mutagenesis on the role of Sp1 in the expression of the pyruvate kinase M and aldolase A genes.10023068
GABPB1UTRNPositive RegulationActivation of utrophin promoter by heregulin via the ets-related transcription factor complex GA-binding protein alpha/beta.10359616
GABPAUTRNPositive RegulationActivation of utrophin promoter by heregulin via the ets-related transcription factor complex GA-binding protein alpha/beta.10359616
IncreasedMycCCND2Neutral RegulationWe now show that p27 is rapidly and transiently sequestered by cyclin D2-Cdk4 complexes upon activation of Myc and that cyclin D2 is a direct target gene of Myc.10508165
IncreasedMycCCND2BindingWe now show that p27 is rapidly and transiently sequestered by cyclin D2-Cdk4 complexes upon activation of Myc and that cyclin D2 is a direct target gene of Myc.10508165
NR1H4FABP6Positive RegulationDeletion and mutation analyses demonstrate that the FXR/RXRalpha heterodimer activates transcription through an inverted repeat bile acid responsive element located in position -160/-148 of the human I-BABP promoter.10514450
RXRAFABP6Positive RegulationDeletion and mutation analyses demonstrate that the FXR/RXRalpha heterodimer activates transcription through an inverted repeat bile acid responsive element located in position -160/-148 of the human I-BABP promoter.10514450
NR1H4FABP6BindingDeletion and mutation analyses demonstrate that the FXR/RXRalpha heterodimer activates transcription through an inverted repeat bile acid responsive element located in position -160/-148 of the human I-BABP promoter.10514450
RXRAFABP6BindingDeletion and mutation analyses demonstrate that the FXR/RXRalpha heterodimer activates transcription through an inverted repeat bile acid responsive element located in position -160/-148 of the human I-BABP promoter.10514450
Sp1BaxNeutral RegulationThe activity of the murine Bax promoter is regulated by Sp1/3 and E-box binding proteins but not by p53.10510469
Sp3BaxNeutral RegulationThe activity of the murine Bax promoter is regulated by Sp1/3 and E-box binding proteins but not by p53.10510469
NR2C1HRH1BindingWe have identified a DNA response element (TR2RE-HR) in the 3' flanking region of the human histamine H1 receptor gene as a target for the TR2 orphan receptor, a member of the steroid/thyroid hormone receptor superfamily.10391141
SP1ABCC8Positive RegulationTogether, these results indicate that SP1 may mediate transcription initiation of the SUR1 gene.10373428
CREB1TNFNeutral RegulationAdditional mutagenesis and transfection studies demonstrated that NF-kappaB and CREB/AP-1 are important regulators of inducible TNF promoter activity in primary human T lymphocytes.10551799
GATA4Adora1Positive RegulationThese observations suggest that embryonic A1AR expression involves activation of the A1AR promoter by GATA-4 and Nkx2.5.10318839
NKX2-5Adora1Positive RegulationThese observations suggest that embryonic A1AR expression involves activation of the A1AR promoter by GATA-4 and Nkx2.5.10318839
RUNX1CR1Positive RegulationAnalysis of transcription activities showed that AML1 increased the CR1 promoter activity and that TEL repressed the basal activity of the promoter.10597047
ETV6CR1Negative RegulationAnalysis of transcription activities showed that AML1 increased the CR1 promoter activity and that TEL repressed the basal activity of the promoter.10597047
ETS1DDIT3Neutral RegulationRegulation of the human stress response gene GADD153 expression: role of ETS1 and FLI-1 gene products.10510472
FLI1DDIT3Neutral RegulationRegulation of the human stress response gene GADD153 expression: role of ETS1 and FLI-1 gene products.10510472
MYCCDH1Positive RegulationHere we show that only a wild type c-myc gene, coding for the two c-Myc proteins c-Myc2 and c-Myc1, was able to transactivate the E-cadherin promoter, in contrast to c-Myc2 or c-Myc1 alone which strongly repressed E-cadherin in both epithelial cells and fibroblasts.10523846
MYCCDH1Negative RegulationHere we show that only a wild type c-myc gene, coding for the two c-Myc proteins c-Myc2 and c-Myc1, was able to transactivate the E-cadherin promoter, in contrast to c-Myc2 or c-Myc1 alone which strongly repressed E-cadherin in both epithelial cells and fibroblasts.10523846
SP3SLC2A1BindingIn summary, we provide direct evidence that the transcription factor Sp3 represses gene expression in non-muscle and muscle cells and this is likely to operate in fetal heart by binding to the GLUT1 gene promoter.10556032
SP3SLC2A1Negative RegulationIn summary, we provide direct evidence that the transcription factor Sp3 represses gene expression in non-muscle and muscle cells and this is likely to operate in fetal heart by binding to the GLUT1 gene promoter.10556032
GABPB1UTRNBindingTaken together, these results suggest that the GA-binding protein alpha/beta complex of transcription factors binds and activates the utrophin promoter in response to heregulin-activated extracellular signal-regulated kinase in muscle cell cultures.10359616
GABPAUTRNBindingTaken together, these results suggest that the GA-binding protein alpha/beta complex of transcription factors binds and activates the utrophin promoter in response to heregulin-activated extracellular signal-regulated kinase in muscle cell cultures.10359616
GABPB1UTRNPositive RegulationTaken together, these results suggest that the GA-binding protein alpha/beta complex of transcription factors binds and activates the utrophin promoter in response to heregulin-activated extracellular signal-regulated kinase in muscle cell cultures.10359616
GABPAUTRNPositive RegulationTaken together, these results suggest that the GA-binding protein alpha/beta complex of transcription factors binds and activates the utrophin promoter in response to heregulin-activated extracellular signal-regulated kinase in muscle cell cultures.10359616
PDX1INSBindingBoth drugs prevented a rise in blood oxidative stress markers (8-hydroxy-2'-deoxyguanosine and malondialdehyde + 4-hydroxy-2-nonenal), and partially prevented hyperglycemia, glucose intolerance, defective insulin secretion as well as decrements in beta cell insulin content, insulin gene expression, and PDX-1 (STF-1) binding to the insulin gene promoter.10485916
IncreasedEtv5IfngPositive RegulationRetroviral expression of ERM did not restore IFN-gamma production in Stat4-deficient T cells, but augmented IFN-gamma expression in Stat4-heterozygous T cells.10097133
IncreasedJUNCGANeutral RegulationThis process is accompanied by a decrease in estrogen receptor alpha gene expression as well as an increase in the expression of transcription factors known to regulate the alpha-subunit promoter, such as cJun and P-LIM.10478837
IncreasedLHX3CGANeutral RegulationThis process is accompanied by a decrease in estrogen receptor alpha gene expression as well as an increase in the expression of transcription factors known to regulate the alpha-subunit promoter, such as cJun and P-LIM.10478837
MYBCCNA1Positive Regulationc-myb transactivates the human cyclin A1 promoter and induces cyclin A1 gene expression.10590070
FOSL1ELNNegative RegulationWestern blot analysis shows that bFGF induces a sustained increase in the steady-state levels of Fra 1, and co-transfection of a Fra 1 expression vector with an elastin promoter reporter construct results in an inhibition of elastin promoter activity.10559225
Sp1CEBPABindingSp1 was shown to bind to a GC box at the 5' end of the C/EBP regulatory element in the C/EBPalpha promoter and, in so doing, to competitively prevent binding to and transactivation of the promoter by the C/EBPs.10373535
Sp1CEBPANegative RegulationSp1 was shown to bind to a GC box at the 5' end of the C/EBP regulatory element in the C/EBPalpha promoter and, in so doing, to competitively prevent binding to and transactivation of the promoter by the C/EBPs.10373535
CEBPBNos2BindingOur results provide evidence that this NF-IL6 binding site is of central importance for maintaining a high transcriptional rate of the iNOS gene after IFN-gamma/LPS stimulation, and that NF-IL6 may cooperate with hypoxia inducible factor-1 in the orchestration of iron-mediated regulation of iNOS.10229861
CEBPBNos2Positive RegulationOur results provide evidence that this NF-IL6 binding site is of central importance for maintaining a high transcriptional rate of the iNOS gene after IFN-gamma/LPS stimulation, and that NF-IL6 may cooperate with hypoxia inducible factor-1 in the orchestration of iron-mediated regulation of iNOS.10229861
CEBPBCCL13BindingAmong the promoter elements potentially regulating MCP-4 gene expression and/or mediating the effects of anti-inflammatory drugs we identified consensus sequences known to interact with nuclear factors like NF-IL6, AP-2, a NF-kappaB like consensus sequence, gamma-interferon- response and YY-1 elements as well as glucocorticoid response elements.10049733
YY1CCL13BindingAmong the promoter elements potentially regulating MCP-4 gene expression and/or mediating the effects of anti-inflammatory drugs we identified consensus sequences known to interact with nuclear factors like NF-IL6, AP-2, a NF-kappaB like consensus sequence, gamma-interferon- response and YY-1 elements as well as glucocorticoid response elements.10049733
CUX1TGFBR2BindingIn this study, we show that the CDP/Cut (CCAAT displacement protein) transcription factor, a transcriptional repressor, binds both the wild type and the mutant TbetaR-II promoter.10545217
Myf5MyogBindingHere we show that transcription of the myogenin gene in differentiated cells correlates with MEF2 and NF1 binding to their cognate sites in the proximal myogenin promoter but not with binding of Myf5 or MyoD to the E-box.10548518
MYOD1MyogBindingHere we show that transcription of the myogenin gene in differentiated cells correlates with MEF2 and NF1 binding to their cognate sites in the proximal myogenin promoter but not with binding of Myf5 or MyoD to the E-box.10548518
CebpbCol1a1Positive RegulationTransforming growth factor beta1 induces the expression of alpha1(I) procollagen mRNA by a hydrogen peroxide-C/EBPbeta-dependent mechanism in rat hepatic stellate cells.10051504
Etv4Ggt1Positive RegulationUsing transient transfection, PEA3 activated transcription of the rat GGT mRNA-IV promoter only in cultured epididymal cells from the rat initial segment, but not in Cos-1 or NRK-52E cells.10026114
JUNCDKN1APositive Regulationc-Jun transactivates the promoter of the human p21(WAF1/Cip1) gene by acting as a superactivator of the ubiquitous transcription factor Sp1.10506225
TFAP2AEGFRBindingHTF can also bind, but with a lower affinity, to a related cis sequence present in the epidermal growth factor receptor promoter.10363966
CEBPAKrt10Positive RegulationIn cultured cells, C/EBPalpha and C/EBPbeta are each capable of activating the k10 promoter via three binding sites, identified by site-directed mutagenesis.10588870
CEBPBKrt10Positive RegulationIn cultured cells, C/EBPalpha and C/EBPbeta are each capable of activating the k10 promoter via three binding sites, identified by site-directed mutagenesis.10588870
CEBPAKrt10BindingIn cultured cells, C/EBPalpha and C/EBPbeta are each capable of activating the k10 promoter via three binding sites, identified by site-directed mutagenesis.10588870
CEBPBKrt10BindingIn cultured cells, C/EBPalpha and C/EBPbeta are each capable of activating the k10 promoter via three binding sites, identified by site-directed mutagenesis.10588870
TP53S100A2Positive RegulationFurthermore, endogenous S100A2 mRNA expression is induced by etoposide in p53 positive, but not in p53 negative cells.10094469
TP53BAXBindingIn addition, the interaction of p53 with its response element from the bax promoter, as compared with its interaction with its element from the p21 promoter, involves a conformationally distinct form of the protein.10559267
TP53CDKN1ABindingIn addition, the interaction of p53 with its response element from the bax promoter, as compared with its interaction with its element from the p21 promoter, involves a conformationally distinct form of the protein.10559267
LRRFIP1EGFRBindingGC factor (GCF) was reported as a transcriptional regulator that binds to a specific GC-rich sequence in the epidermal growth factor receptor (EGFR) gene promoter and represses its expression.10500253
LRRFIP1EGFRNegative RegulationGC factor (GCF) was reported as a transcriptional regulator that binds to a specific GC-rich sequence in the epidermal growth factor receptor (EGFR) gene promoter and represses its expression.10500253
GCFC2EGFRBindingGC factor (GCF) was reported as a transcriptional regulator that binds to a specific GC-rich sequence in the epidermal growth factor receptor (EGFR) gene promoter and represses its expression.10500253
GCFC2EGFRNegative RegulationGC factor (GCF) was reported as a transcriptional regulator that binds to a specific GC-rich sequence in the epidermal growth factor receptor (EGFR) gene promoter and represses its expression.10500253
IncreasedIRF1SLPINegative RegulationSecretory leukocyte protease inhibitor (SLPI) - a regulator of inflammation and an inhibitor of the LPS response - was identified as a gene repressed after doxycycline induced IRF-1 expression.10498899
MutantSREBF1APOA2BindingSREBP-1 mutants lacking the activation domain bind to their cognate sites and directly repress the apoA-II promoter whereas mutants defective in DNA binding indirectly repress the apoA-II promoter activity, possibly by a squelching mechanism.10364076
MutantSREBF1APOA2Negative RegulationSREBP-1 mutants lacking the activation domain bind to their cognate sites and directly repress the apoA-II promoter whereas mutants defective in DNA binding indirectly repress the apoA-II promoter activity, possibly by a squelching mechanism.10364076
MutantSREBF1APOA2Negative RegulationSREBP-1 mutants lacking the activation domain bind to their cognate sites and directly repress the apoA-II promoter whereas mutants defective in DNA binding indirectly repress the apoA-II promoter activity, possibly by a squelching mechanism.10364076
IncreasedCEBPBCXCL8Positive RegulationTaken together these studies indicate that TNF-alpha-induced redox changes in lung epithelial cells are responsible for the transcriptional activation of IL-8 and that coordinate activation of NF-kappaB and NF-IL-6 mediate the response.10462040
RELAFASNeutral RegulationActivation-dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment.10022897
STAT6CCL11BindingElectrophoretic mobility shift assays revealed NF-kappa B binding after TNF-alpha stimulation and STAT6 binding after IL-4 stimulation using a DNA probe derived from the eotaxin promoter.10586089
SP1CLCN2Positive RegulationThis work suggests that Sp1 and Sp3 activate ClC-2 gene transcription and that reduction in Sp1 and Sp3 at birth explains perinatal downregulation of ClC-2 in the lung.10198359
Sp3CLCN2Positive RegulationThis work suggests that Sp1 and Sp3 activate ClC-2 gene transcription and that reduction in Sp1 and Sp3 at birth explains perinatal downregulation of ClC-2 in the lung.10198359
NCOR1TRHPositive RegulationTR and RAR can similarly interact with the corepressor proteins, and the cotransfected nuclear receptor corepressor (N-CoR) has been demonstrated to augment the transcriptional stimulation of the TRH gene by unliganded TR.10537125
HNF4AALDH2Positive RegulationActivity of the human aldehyde dehydrogenase 2 promoter is influenced by the balance between activation by hepatocyte nuclear factor 4 and repression by perosixome proliferator activated receptor delta, chicken ovalbumin upstream promoter-transcription factor, and apolipoprotein regulatory protein-1.10352676
PPARDALDH2Negative RegulationActivity of the human aldehyde dehydrogenase 2 promoter is influenced by the balance between activation by hepatocyte nuclear factor 4 and repression by perosixome proliferator activated receptor delta, chicken ovalbumin upstream promoter-transcription factor, and apolipoprotein regulatory protein-1.10352676
NR2F2ALDH2Negative RegulationActivity of the human aldehyde dehydrogenase 2 promoter is influenced by the balance between activation by hepatocyte nuclear factor 4 and repression by perosixome proliferator activated receptor delta, chicken ovalbumin upstream promoter-transcription factor, and apolipoprotein regulatory protein-1.10352676
LITAFTNFPositive RegulationTogether, these findings suggest that LITAF plays an important role in the activation of the human TNF-alpha gene and proposes a new mechanism to control TNF-alpha gene expression.10200294
IncreasedMsx1Msx1Negative RegulationTo investigate the molecular mechanism by which Msx1 represses the activity of its own promoter, we transfected C2C12 myoblasts with Msx1-promoter-luciferase constructs and assessed reporter gene activity, with and without the exogenous expression of Msx1.10215616
ATF2JUNBindingSupershift assays revealed that, during the early induction phase of the c-jun gene, the proximal activator protein-1 (jun1) site of the c-jun promoter was mainly occupied by the constitutively expressed activating transcription factor-2, whereas the late induction correlated with the predominant binding of c-Jun and, to a lesser extent, activating transcription factor-2 to the distal activator protein-1 (jun2) site.10366004
JUNJUNBindingSupershift assays revealed that, during the early induction phase of the c-jun gene, the proximal activator protein-1 (jun1) site of the c-jun promoter was mainly occupied by the constitutively expressed activating transcription factor-2, whereas the late induction correlated with the predominant binding of c-Jun and, to a lesser extent, activating transcription factor-2 to the distal activator protein-1 (jun2) site.10366004
NEUROD1NEUROD1Positive RegulationThese results raised the possibility that the NeuroD gene expression is positively regulated through the E box sequence, not only by NeuroD itself but also by another E box binding protein.10366743
MutantTP53CDKN1APositive RegulationFurthermore, we found that deletion of the proline-rich region has no or very mild effects on activation of several transiently transfected p53 target gene promoters, i.e., the p21, MDM2, BAX, and GADD45 promoters.10321740
MutantTP53MDM2Positive RegulationFurthermore, we found that deletion of the proline-rich region has no or very mild effects on activation of several transiently transfected p53 target gene promoters, i.e., the p21, MDM2, BAX, and GADD45 promoters.10321740
MutantTP53BAXPositive RegulationFurthermore, we found that deletion of the proline-rich region has no or very mild effects on activation of several transiently transfected p53 target gene promoters, i.e., the p21, MDM2, BAX, and GADD45 promoters.10321740
MutantTP53GADD45APositive RegulationFurthermore, we found that deletion of the proline-rich region has no or very mild effects on activation of several transiently transfected p53 target gene promoters, i.e., the p21, MDM2, BAX, and GADD45 promoters.10321740
ReducedNFATC2IL4BindingEvidence for suppressed activity of the transcription factor NFAT1 at its proximal binding element P0 in the IL-4 promoter associated with enhanced IL-4 gene transcription in T cells of atopic patients.10069428
TP53BAXNeutral RegulationSpecifically, we show that expression of alpha6beta4 in carcinoma cells that lack this integrin stimulates an increase in the transactivating function of p53 as demonstrated by the ability of this integrin to up-regulate the expression of a p53-sensitive reporter gene as well as the endogenous p53 response gene, bax.10400708
IncreasedKLF11HBG2Positive RegulationTransfection of K562 cells with FKLF cDNA enhanced the expression of the endogenous epsilon- and gamma-globin genes, suggesting an in vivo role of FKLF in fetal and embryonic globin gene expression.10207080
IncreasedKLF11HBE1Positive RegulationTransfection of K562 cells with FKLF cDNA enhanced the expression of the endogenous epsilon- and gamma-globin genes, suggesting an in vivo role of FKLF in fetal and embryonic globin gene expression.10207080
IncreasedKLF11HBG1Positive RegulationTransfection of K562 cells with FKLF cDNA enhanced the expression of the endogenous epsilon- and gamma-globin genes, suggesting an in vivo role of FKLF in fetal and embryonic globin gene expression.10207080
IncreasedNr2f2PklrNegative RegulationOverexpression of COUP-TFII abrogated the USF-dependent transactivation of an artificial GlRE-dependent promoter in COS cells and the glucose responsiveness of the L-PK promoter in hepatocytes in primary culture.10497199
ReducedFOSCLUNegative RegulationHere, we demonstrate, by supershift analysis, that JunB, JunD, Fra1, Fra2, and c-Fos bound to AP-1 but that prior treatment of the cells with TGFbeta reduced dramatically c-Fos binding, suggesting that c-Fos might be playing a negative regulatory role in clusterin gene expression.10406964
SREBF1APOA2Positive RegulationDespite the strong transactivation of the apoA-II promoter by SREBP-1 we could not demonstrate significant changes on the endogenous apoA-II mRNA levels of HepG2 cells after cotransfection with SREBP-1 or in the presence or absence of cholesterol and 25-OH-cholesterol.10364076
SPI1CSF2RBPositive RegulationWe further show that PU.1 has to cooperate with C/EBP proteins to induce beta c transcription.10453008
Etv4Ggt1Neutral RegulationThis suggests that PEA3 may be involved in regulating transcription from the rat GGT mRNA-IV gene promoter in the initial segment.10026114
TP53CCNB1Neutral Regulationp53 regulates a G2 checkpoint through cyclin B1.10051609
SRFEGR1BindingWe conclude that GH stimulates transcription of egr-1 primarily through activation of these Ets factors at multiple sites on the promoter and that stabilization of ternary complexes with SRF at these sites maximizes this response.10194767
SRFEGR1Positive RegulationWe conclude that GH stimulates transcription of egr-1 primarily through activation of these Ets factors at multiple sites on the promoter and that stabilization of ternary complexes with SRF at these sites maximizes this response.10194767
IncreasedNFKB2TNFNegative RegulationAlso, overexpression of p52 will suppress activity of TNF promoter reporter gene constructs.10595582
TEAD1TGFABindingWe also show that the spacing between the AP-2 and the TEF1 sites in the TGF-alpha promoter has little effect on the transcription from the TGF-alpha promoter.10076050
Phox2aDBHPositive RegulationTogether with our previous studies suggesting a critical role of Phox2a for noradrenergic-specific expression of the DBH gene, these data support a model in which distinct, or partially distinct, molecular mechanisms regulate cell-specific expression of the NET and DBH genes.10037744
DLX4MYCNeutral RegulationThese results suggest that DLX-7 may regulate c-myc and GATA-1 genes at transcriptional and post-transcriptional levels.10402242
DLX4GATA1Neutral RegulationThese results suggest that DLX-7 may regulate c-myc and GATA-1 genes at transcriptional and post-transcriptional levels.10402242
HSF1IL1BBindingAt least in the case of the IL1B promoter, repression did not seem to involve another factor whose activity is affected by the NSAIDs, NFkappaB as the IL1B promoter fragment used in our studies is not NFkappaB responsive and binds specifically to HSF1.10328874
TP53CDK1Negative Regulationp53 negatively regulates cdc2 transcription via the CCAAT-binding NF-Y transcription factor.10514438
HNF4AANGBindingIn vitro assays indicated that HNF4 bound to the promoter elements and strongly activated the ANG transcription, but that chicken ovalbumin upstream promoter transcription factor (COUP-TF), a transcriptional repressor, dramatically repressed human ANG transcription through the promoter elements and the downstream enhancer core elements.10574924
HNF4AANGPositive RegulationIn vitro assays indicated that HNF4 bound to the promoter elements and strongly activated the ANG transcription, but that chicken ovalbumin upstream promoter transcription factor (COUP-TF), a transcriptional repressor, dramatically repressed human ANG transcription through the promoter elements and the downstream enhancer core elements.10574924
NR2F1ANGNegative RegulationIn vitro assays indicated that HNF4 bound to the promoter elements and strongly activated the ANG transcription, but that chicken ovalbumin upstream promoter transcription factor (COUP-TF), a transcriptional repressor, dramatically repressed human ANG transcription through the promoter elements and the downstream enhancer core elements.10574924
Stat6IGHEBindingStat1, which is activated by IFN-gamma, cannot recognize the Stat6-specific IL-4 response element in the epsilon promoter.10201928
CREB1CREBBPBindingThe inhibitory effect of NFI on transcription of the PEPCK gene induced by the catalytic subunit of protein kinase A appears to be the result of an interaction between NFI and the CREB-binding protein in which NFI competes with CREB for binding to the CREB-binding site on CBP.10085123
GATA1EPB42Positive RegulationTaken together, these results suggest that the erythroid transcription factor GATA-1 plays an important role in the induction of P4.2 gene expression during erythroid cell differentiation.10395794
NR3C1SERPINE1Negative RegulationHere we report that glucocorticoid receptor (GR) represses TGF-beta transcriptional activation of the type-1 plasminogen activator inhibitor (PAI-1) gene in a ligand-dependent manner.10518526
POU1F1PRLPositive RegulationFurthermore, CBP also increases the activation of the PRL promoter by GHF-1 and the ligand-independent activation by both wild-type and mutant VDR.10406465
VDRPRLPositive RegulationFurthermore, CBP also increases the activation of the PRL promoter by GHF-1 and the ligand-independent activation by both wild-type and mutant VDR.10406465
MutantVDRPRLPositive RegulationFurthermore, CBP also increases the activation of the PRL promoter by GHF-1 and the ligand-independent activation by both wild-type and mutant VDR.10406465
EBF1BlkNeutral RegulationThese data indicate that EBF plays an important role in the regulation of the Blk promoter in early B cell development and that EBF and BSAP are capable to act in cooperation to induce a target gene.10553071